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KMID : 0381919940240010102
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Korean Journal of Microscopy
1994 Volume.24 No. 1 p.102 ~ p.113
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Structural Character of Two-Dimensional Crystal of the S-Layer Glycoprotein from Methanoplanus limicola
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정강원/Cheong, Gang Won
과대오/주우홍/Kwack, Dae Oh/Joo, Woo Hong
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Abstract
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A thin section of the methanogenic archaebacterium, Methanoplanus limicola, shows that the surface glycoprotein array (S-layer) is separated from plasma membrane by a distinct interspace, approximately 4.0-6.Onm wide. We report our structural study of the S-layer by electron crystallographic techniques. Image analysis and thin sections of the S-layer treated with and without triton disclosed that retention of the single layer crystal requires the presence of detergent to avoid hydrophobic bonding.
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